Using a Multi-Compartment Device With a Microgroove Barrier

Using a multi-compartment device with a microgroove barrier is a novel technique for evaluating axon regeneration. This technology permits axon removal without impacting the somatic compartment. For example, a 50-in. (1.27-m) XGA PDP is a good candidate for this test. A device with a microgroove barrier allows the researcher to study the regeneration potential of injured hSC axons.

Microgroove barriers provide a fluidic isolation layer between the axons and cell bodies, allowing other cellular growths to pass through them. The 450-um device is the most popular microfluidic device available, and is pre-assembled and offers fluidic isolation. A 100-mm Xona device is also available. The 900-um version uses the same type of barrier, but can handle dendrites. Read more!

The 900-um device is ideal for long-term experiments, culture organization, and fluidic isolation. Although the 450-um barrier may be a better choice for many applications, dendrites will still be able to cross it after two weeks. The wide microgrooves also enable the diversion of flow around the post, which changes the streamline patterns. A 900-um microgroove device can be used for experiments with long processes and asymmetrical membrane.

Using a 450-um microgroove barrier is suitable for culture organization and long-term experiments. Its narrow ridges allow for nutrient exchange between compartments while minimizing evaporation. The 450-um microgroove barrier also provides adequate fluidic isolation and culture organization for neuronal cultures. A 450-mm device is a suitable choice for studies of dendrites and axons.

In vitro culture, a microgroove-barrier system provides optimal conditions for the separation of axons and dendrites. Axons and dendrites are separated using a 450-mm device. The 450-mm device is designed for both culture organization and fluidic isolation. Its axons are 2.5-mm wide. The axons are about 1.5-mm wide. You may discover more here.

Another microgroove-barrier device, the 900-um, has a 450-um microgroove barrier. The 900-um barrier has the ability to separate neuronal cells from each other in two-dimensional culture. The 450-um barrier is not suitable for long-term experimentation. The 900-um microgroove barrier is ideal for studies involving neurons that have long processes.

In addition to its use in vivo research, a microgroove barrier has numerous advantages. This type of device is highly flexible, allowing various microscopy techniques to be carried out. In contrast, conventional processes have disadvantages. It is not feasible to transfer biomolecules through a poly-DMS barrier. It is possible to transfer different chemicals to different parts of a tissue. This type of microgroove barrier also facilitates the use of chemical solutions in a cell culture.

In a microfluidic device, a top piece is lifted to access neurons. The corresponding bottom piece contains a solid physical barrier. In the microgroove-containing chamber, neurons are positioned inside the top portion. The corresponding side wall microgrooves have a cellular substrate and a solid physical barrier. The two components can be polarized and evaluated in an open culture. The resulting cells can be manipulated in the same way as in a liquid-tight sphere. Check out some facts at

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